초록
<P><B>Abstract</B></P> <P>Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignification by 1.2 and 1.5-fold respectively, compared to conventional bio-pretreatment due to inhibition of 61% of cellulase activity in IMBP. Further increase in NCI concentration showed negative effect on <I>Pleurotus florida</I> growth and suppress the laccase productivity by 1.1-fold. Laccase activity in IMBP was found to be 2.0U/mL on 19<SUP>th</SUP> day, which is higher than (1.5U/mL) conventional bio-pretreatment. Physico-chemical modifications in paddy straw before and after pretreatment were analysed by SEM, ATR-FTIR, XRD and TGA. According to these findings, the IMBP technology can be a viable eco-friendly technology for sustainable production of bioethanol with maximum cellulose recovery.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Paddy straw was pretreated using inhibitor mediated biological pretreatment (IMBP). </LI> <LI> IMBP and control pretreatment were compared for efficiency in cellulose recovery. </LI> <LI> 8% Natural Cellulase Inhibitor (NCI) showed 1.2-fold improved cellulose recovery. </LI> <LI> NCI, improved laccase productivity up to 23% compared to control. </LI> <LI> Increasing NCI concentration beyond 8% showed suppression of fungal growth. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>