초록
<P><B>Abstract</B></P> <P>An effective ferulic acid degrading bacterium, <I>Escherichia</I> sp. HHQ-1, was added to alkali-pretreated rice straw degrading system to <I>in-situ</I> detoxify the ferulic acid inhibitors existed in the hydrolysates. It was shown that the production of reducing sugars in <I>Escherichia</I> sp. HHQ-1-<I>Trichoderma reesei</I> consortia degrading system could achieve 221.33 mg L<SUP>−1</SUP> at 60 h, which was 7.84% higher than that in single degrading system. All the three main cellulases were more stable in consortia degrading system, and the activity of β-glucosidase was 4.23 fold higher than that in single degrading system. Besides, it was supposed that cell membrane was more integrally protected in consortia degrading system. Scanning Electron Microscope (SEM), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectrometer (FTIR) were used to observe the morphological changes of rice straw biomass. It was indicated that <I>in-situ</I> detoxification could severely destroy basic tissue of rice straw, dramatically decrease crystallinity index of crystalline region and effectively degrade refractory-degraded lignin parts of lignocellulose. Compared to the single degrading system, the consortia degrading system could <I>in-situ</I> detoxify the main inhibitors which existed in pretreated rice straw hydrolysates, realize simultaneous pretreatment and detoxification, thus increasing the bioethanol yield and reducing the cost of bioethanol production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In-situ detoxification was successfully realized for bioethanol production. </LI> <LI> In-situ detoxification increased the production of reducing sugars effectively. </LI> <LI> Mechanisms of the stimulatory effect of in-situ detoxification were investigated. </LI> <LI> Morphology changes of rice straw were analyzed by SEM, XRD and FTIR. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>