초록
<P><I>Dekkera bruxellensis</I> is a non-conventional Crabtree-positive yeast with a good ethanol production capability. Compared to <I>Saccharomyces cerevisiae</I>, its tolerance to acidic pH and its utilization of alternative carbon sources make it a promising organism for producing biofuel. In this study, we developed an auxotrophic transformation system and an expression vector, which enabled the manipulation of <I>D. bruxellensis</I>, thereby improving its fermentative performance. Its gene <I>ADH3</I>, coding for alcohol dehydrogenase, was cloned and overexpressed under the control of the strong and constitutive promoter <I>TEF1</I>. Our recombinant <I>D. bruxellensis</I> strain displayed 1.4 and 1.7 times faster specific glucose consumption rate during aerobic and anaerobic glucose fermentations, respectively; it yielded 1.2 times and 1.5 times more ethanol than did the parental strain under aerobic and anaerobic conditions, respectively. The overexpression of <I>ADH3</I> in <I>D. bruxellensis</I> also reduced the inhibition of fermentation by anaerobiosis, the “Custer effect”. Thus, the fermentative capacity of <I>D. bruxellensis</I> could be further improved by metabolic engineering.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1007/s00253-015-7266-x) contains supplementary material, which is available to authorized users.</P>