BioChem - DOS

Enhanced saccharification of rice straw using hypochlorite-hydrogen peroxide

Biotechnology and bioprocess engineering : Bbe

Kang, Hee-Kyoung; Kim, Na-Hyun M.; Kim, Gha-Hyun J.; Seo, Eun-Seong; Ryu, Hwa-Ja; Yun, Sang-Il; Choi, Hyun-Chul; Day, Donal F.; Kim, Jong-Ho; Cho, Dong-Lyun; Kim, Do-Man

Rice straw is a lignocellulosic biomass, and has been recognized as a renewable organic substance and alternative energy source. In this study, rice straw was pretreated with hypochlorite-hydrogen peroxide (Ox-B) solution. The optimal pretreatment conditions were determined via response surface methodology, and the pretreated rice straw was hydrolyzed with exo-glucanase, end-oglucanase, hemicellulase, and <TEX>${\beta}$</TEX>-glucosidase Accellerase <TEX>$1000^{TM}$</TEX> (endo-glucanase equivalent activity of 1,250 carboxy methyl cellulose (CMC) U/g of rice straw pretreated for 24 h). The optimal conditions were as follows: 60 min pretreatment using Ox-B solution containing 0.6% hypochlorite and 25% hydrogen peroxide for 1 g of rice straw in a total reaction volume of 240 mL. Under these conditions, 406.8 mg of D-glucose and 224.0 mg of D-xylose were obtained from 1 g of rice straw. The fermentation of enzymatic hydrolysates containing 8.14 g/L D-glucose and 4.49 g/L D-xylose with Pichia stipitis generated 3.65 g/L of ethanol with a corresponding yield of 0.37 g/g. The maximum possible ethanol conversion rate is 72.54%.

2011

Korean Society for Biotechnology and Bioengineering

1226-8372

1976-3816

16

2

pp.273-281

10.1007/s12257-010-0262-1

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=JAKO201120241363282

pretreatment; ethanol; hypochlorite-hydrogen peroxide; lignocelluloses; Pichia stipitis