Phenotypic characterisation of Saccharomyces spp. yeast for tolerance to stresses encountered during fermentation of lignocellulosic residues to produce bioethanol
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Phenotypic characterisation of Saccharomyces spp. yeast for tolerance to stresses encountered during fermentation of lignocellulosic residues to produce bioethanol
학술지
Microbial cell factories
저자명
Wimalasena, Tithira T; Greetham, Darren; Marvin, Marcus E; Liti, Gianni; Chandelia, Yogeshwar; Hart, Andrew; Louis, Edward J; Phister, Trevor G; Tucker, Gregory A; Smart, Katherine A
초록
<P><B>Background</B></P><P>During industrial fermentation of lignocellulose residues to produce bioethanol, microorganisms are exposed to a number of factors that influence productivity. These include inhibitory compounds produced by the pre-treatment processes required to release constituent carbohydrates from biomass feed-stocks and during fermentation, exposure of the organisms to stressful conditions. In addition, for lignocellulosic bioethanol production, conversion of both pentose and hexose sugars is a pre-requisite for fermentative organisms for efficient and complete conversion. All these factors are important to maximise industrial efficiency, productivity and profit margins in order to make second-generation bioethanol an economically viable alternative to fossil fuels for future transport needs.</P><P><B>Results</B></P><P>The aim of the current study was to assess <I>Saccharomyces</I> yeasts for their capacity to tolerate osmotic, temperature and ethanol stresses and inhibitors that might typically be released during steam explosion of wheat straw. Phenotypic microarray analysis was used to measure tolerance as a function of growth and metabolic activity. <I>Saccharomyces</I> strains analysed in this study displayed natural variation to each stress condition common in bioethanol fermentations. In addition, many strains displayed tolerance to more than one stress, such as inhibitor tolerance combined with fermentation stresses.</P><P><B>Conclusions</B></P><P>Our results suggest that this study could identify a potential candidate strain or strains for efficient second generation bioethanol production. Knowledge of the <I>Saccharomyces</I> spp. strains grown in these conditions will aid the development of breeding programmes in order to generate more efficient strains for industrial fermentations.</P>