초록
<P><B>Abstract</B></P> <P>Cellulosic ethanol production relies on the biochemical (enzymatic) conversion of lignocellulose to fermentable sugars and ultimately to bioethanol. However, the cost of lignocellulolytic enzymes is a limiting factor in the commercialisation of this technology. This therefore necessitates the optimisation of lignocellulolytic enzyme cocktails through the elucidation of synergistic interactions between enzymes so as to improve lignocellulose hydrolysis and also lower protein loadings in these reactions. However, many factors affect the synergism that occurs between these lignocellulolytic enzymes, such as enzyme ratios, substrate characteristics, substrate loadings, enzyme loadings and time. This review examines the effect of time on the synergistic dynamics between lignocellulolytic enzymes during the hydrolysis of both complex (true) lignocellulosic substrates and model substrates. The effect of <I>sequential</I> and <I>simultaneous</I> application of the lignocellulolytic enzymes on the synergistic dynamics during the hydrolysis of these substrates is also explored in this review. Finally, approaches are further proposed for efficient and synergistic hydrolysis of both complex lignocellulosic substrates and model substrates. With respect to the synergistic enzymatic hydrolysis of lignocellulosic biomass, this review exposed knowledge gaps that should be covered in future work in order to fully understand how enzyme synergism works: e.g. elucidating protein to protein interactions that exist between these enzymes in establishing synergy; and the effect of lignocellulose degradation products of one enzyme on the behaviour of the other enzyme and ultimately their synergistic relationship.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Time affects the synergistic hydrolysis of lignocellulose by enzymes. </LI> <LI> Elucidation of the choice of enzymes is the key to establishing synergy. </LI> <LI> <I>Simultaneous</I> employment of enzymes is proposed to depolymerize various components in complex lignocellulose. </LI> <LI> <I>Sequential</I> application of enzymes is proposed to hydrolyse the same polysaccharide. </LI> <LI> Application of enzymes may be highly dependent on their properties. </LI> </UL> </P>