초록
<P>Effect of by-products from wet-oxidation explosion, such as formic acid, acetic acid, and furfural on the growth and fermentation, glycolysis and energy metabolism, cytomembrane integrality of<I>Saccharomyces cerevisiae</I>were studied. The results showed that the maximum tolerated concentra tion of<I>S. cerevisiae</I>was 1.8 g/L formic acid , 6.0 g/L acetic acid,2.5g/ furfural, respectively. The inhibition strengths of the typical inhibitors to ethanol fermentation were in the order of formic acid , acetic acid , furfural. When the concentration of these typical inhibitors is 1×IC80, acetic acid has the minimum impact on glycolysis and energy metabolism . When the concentration of these typical inhibitors was 2×IC80, furfural had minimum impact on glycolysis and energy metabolism. However , formic acid can inhibit strongly the glycolysis and energy metabolism of<I>Saccharomyces cerevisiae</I>with any concentration . When compared with ethanol, the impact of these typical inhibitors on<I>S. cerevisiae</I>'s cytomembrane integrality was not very significant. When the concentration of these typical inhibitors varied from 1×IC80to 3×IC80, the results of the leak of Mg<SUP>2+</SUP>was 11%-20% formic acid, 5%-12% acetic acid, 4.5%-8.4% furfural, respectively. However, the result of ethanol that leaded to the leak of Mg<SUP>2+</SUP>was 55%.</P>