초록
<P><B>Abstract</B></P> <P>A multipurpose magnetic nanobiocatalyst is developed by conjugating Pectinex 3XL (a commercial enzyme containing pectinase, xylanase and cellulase activities) on 3-aminopropyl triethoxysilane activated magnetic nanoparticles. The nanobiocatalyst retained 87% of pectinase, 69% of xylanase and 58% of cellulase activity after conjugation on modified nanoparticles as compared to their soluble counterparts. Thermal stability data at 70°C showed increase in enzyme stability after conjugation to nanoparticles and the kinetic parameters (K<SUB>m</SUB> and V<SUB>max</SUB>) remain unaltered after immobilization. The immobilized enzyme system can be successfully used upto 5th cycle after that slight decrease in enzyme activities was observed. The nanobiocatalyst retained high pectinase activities in organic solvents and chemical reagents as compared to free enzymes. DLS data shows that the nanoparticles size increases from 63nm to 86nm after immobilization. Atomic Force Microscopy data confirms the deposition of enzymes on the nanoparticles. The nanobiocatalyst was used for the clarification of pine apple and orange juice and was also used for the production of bioethanol. Hydrolysis of pretreated wheat straw produced 1.39g/l and 1.59g/l after treatment with free Pectinex 3xL and nanobiocatalyst respectively. The concentration of bioethanol also increases by 1.4 fold as compared to the free enzyme.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A magnetic nanobiocatalyst of cellulase and hemicellulase was developed. </LI> <LI> Efficiently hydrolyzed insoluble cellulose, pectin and xylan. </LI> <LI> Enhanced stability of enzymes after conjugation with magnetic nanoparticles. </LI> <LI> Nanobiocatalyst can be used successfully upto to 5th cycle. </LI> <LI> The nanobiocatalyst was used for the clarification of pineapple and orange juices and was also used for the production of bioethanol from wheat straw. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>