초록
<P>The effect of different pretreatment methods, temperature, and enzyme concentration on ethanol production from 8 lignocellulosic agrowaste by simultaneous saccharification and fermentation (SSF) using recombinant cellulase and <I>Saccharomyces cerevisiae</I> were studied. Recombinant cellulase was isolated from <I>E. coli</I> BL21 cells transformed with C<I>t</I>Lic26A-Cel5-CBM11 full-length gene from <I>Clostridium thermocellum</I> and produced in both batch and fed-batch processes. The maximum cell OD and specific activity in batch mode were 1.6 and 1.91 U/mg, respectively, whereas in the fed-batch mode, maximum cell OD and specific activity were 3.8 and 3.5 U/mg, respectively, displaying a 2-fold increase. Eight substrates, <I>Syzygium cumini</I> (jamun), <I>Azadirachta indica</I> (neem), <I>Saracens indica</I> (asoka), <I>bambusa dendrocalmus</I> (bamboo), <I>Populas nigra</I> (poplar), <I>Achnatherum hymenoides</I> (wild grass), <I>Eucalyptus marginata</I> (eucalyptus), and <I>Mangifera indica</I> (mango), were subjected to SSF. Of three pretreatments, acid, alkali, and steam explosion, acid pretreatment <I>Syzygium cumini</I> (Jamun) at 30°C gave maximum ethanol yield of 1.42 g/L.</P>