<P><B>Abstract</B></P> <P>Enzyme recycling by re-adsorption is one of the primary methods for reducing enzyme usage in lignocellulose conversion. This work proposes the combination of an engineered yeast strain that expresses β-glucosidase with enzyme recycling to reduce the amount of supplemented β-glucosidase in enzyme recycling experiments. Using the engineered strain, a slight increase in ethanol concentration was obtained after a 96-h fermentation of pretreated corncobs. Ethanol concentrations increased by 34.7% and 62.7% in the following two recycle rounds using the engineered strain compared with those using its parental strain without β-glucosidase addition. Furthermore, with the addition of β-glucosidase at 30CBU/g cellulose, the ethanol concentration after two recycle rounds exceeded 90% of that observed in the first SSF round with the engineered strain at a high initial cellulase loading of 45FPU/g cellulose.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cellulase recycling by re-adsorption was combined with the use of engineered yeast. </LI> <LI> The engineered yeast expressing-β-glucosidase improved ethanol production. </LI> <LI> Cellulase recovery was compared between engineered strain and its parental strain. </LI> <LI> Ethanol production in first recycle round increased by 34.7% by engineered yeast. </LI> <LI> Supplementation of β-glucosidase was reduced using engineered yeast. </LI> </UL> </P>