Bioethanol Production from Xylose-Rich Hydrolysate by Immobilized Recombinant Saccharomyces cerevisiae in Fixed-Bed Reactor
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Bioethanol Production from Xylose-Rich Hydrolysate by Immobilized Recombinant Saccharomyces cerevisiae in Fixed-Bed Reactor
학술지
Industrial biotechnology
저자명
Milessi, Thais S.; Zangirolami, Teresa C.; Perez, Caroline L.; Sandri, Juliana P.; Corradini, Felipe A.S.; Foulquié -Moreno, Maria R.; Thevelein, Johan M.; Giordano, Roberto C.; Giordano, Raquel L.C.
초록
<P>Currently, xylose from biomass hemicellulose is still underused, and the fermentation of crude hemicellulose hydrolysates is a challenge due to the presence of inhibitors. Cell immobilization coupled with fixed-bed reactor could create process conditions for high productivity and yields. Thus, in this work, ethanol production in a fixed-bed reactor loaded with Ca-alginate immobilized recombinant <I>Saccharomyces cerevisiae</I> using undetoxified sugarcane bagasse hemicellulose hydrolysate as substrate was studied. Fermentation was carried out in fixed-bed reactor with 100 mL of reaction volume containing 25g of beads (50 g/L of dry cells) at 35°C, pH 5.2. After 4 h, all fermentable sugars were consumed, with low xylitol formation, leading to ethanol yield of 0.39 g/g and productivity of 4.4 g/L.h. This is a promising strategy with potential to implement process operation under repeated batch or continuous mode and therefore increase the chances of achieving economic viability for 2G ethanol production.</P>