초록
<P><B>Abstract</B></P><P><B>Aims</B></P><P>To investigate multiple tolerance of <I>Saccharomyces cerevisiae</I> obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production.</P><P><B>Methods and Results</B></P><P>After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l<SUP>−1</SUP>) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze‐thawing cycles. Also, Y8A was able to maintain redox homeostasis under oxidative stress, whereas the isogenic parental strain (Y8) could not, indicating higher basal activity levels of antioxidative enzyme Catalase (CAT) and Gluthatione S‐transferase (GST) in Y8A. Y8A reached higher bioethanol levels in a fermentation medium containing up to 8 g l<SUP>−1</SUP> of acetic acid when compared to parental strain Y8.</P><P><B>Conclusions</B></P><P>A multiple‐stress‐tolerant clone was obtained using adaptive evolution in acetic acid. Stress cross‐tolerance could be explained by its enzymatic antioxidative capacity, namely CAT and GST.</P><P><B>Significance and Impact of the Study</B></P><P>We demonstrate that adaptive evolution used in <I>S. cerevisiae</I> was a useful strategy to obtain a yeast clone tolerant to multiple stresses. At the same time, our findings support the idea that tolerance to oxidative stress is the common basis for stress cotolerance, which is related to an increase in the specific enzymes CAT and GST but not in Superoxide dismutase, emphasizing the fact that detoxification of H<SUB>2</SUB>O<SUB>2</SUB> and not O<SUB>2</SUB>˙ is a key condition for multiple stress tolerance in <I>S. cerevisiae</I>.</P>