BioChem - DOS

Development of rapid bioconversion with integrated recycle technology for ethanol production from extractive ammonia pretreated corn stover

Biotechnology and bioengineering

Jin, Mingjie; Liu, Yanping; da Costa Sousa, Leonardo; Dale, Bruce E.; Balan, Venkatesh

<P><B>ABSTRACT</B></P><P>High enzyme loading and low productivity are two major issues impeding low cost ethanol production from lignocellulosic biomass. This work applied rapid bioconversion with integrated recycle technology (RaBIT) and extractive ammonia (EA) pretreatment for conversion of corn stover (CS) to ethanol at high solids loading. Enzymes were recycled via recycling unhydrolyzed solids. Enzymatic hydrolysis with recycled enzymes and fermentation with recycled yeast cells were studied. Both enzymatic hydrolysis time and fermentation time were shortened to 24 h. Ethanol productivity was enhanced by two times and enzyme loading was reduced by 30%. Glucan and xylan conversions reached as high as 98% with an enzyme loading of as low as 8.4 mg protein per g glucan. The overall ethanol yield was 227 g ethanol/kg EA&#8208;CS (191 g ethanol/kg untreated CS). Biotechnol. Bioeng. 2017;114: 1713&ndash;1720. &copy; 2017 Wiley Periodicals, Inc.</P>

2017

0006-3592

1097-0290

114

8

pp.1713-1720

10.1002/bit.26302

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78086684

lignocellulosic ethanol; RaBIT process; EA pretreatment; enzymatic hydrolysis; enzyme recycling; ethanol fermentation