초록
<P><B>Abstract</B></P> <P>The mixture of <I>Eucalyptus globulus</I> wood (EGW) and cheese whey powder (CWP) was proposed for intensification of simultaneous saccharification and fermentation (SSF) at high temperature and solid loadings using the industrial <I>Saccharomyces cerevisiae</I> Ethanol Red® strain. High ethanol concentration (93 g/L), corresponding to 94% ethanol yield, was obtained at 35 °C from 37% of solid mixture using cellulase and β-galactosidase enzymes (24.2 FPU/g and 20.0 U/g, respectively). The use of CWP mixed with pretreated EGW increased the ethanol concentration in 1.5-fold, in comparison with SSF experiments without CWP for both Ethanol Red® and CEN.PK113-7D strains. Moreover, 1.4-fold higher ethanol concentration was obtained with Ethanol Red®, in comparison with CEN.PK113-7D strain. Ethanol Red® strain was genetically engineered for β-galactosidase production in order to advance towards a fully integrated process. This work shows the feasibility of attaining high ethanol concentrations in second generation bioprocesses by a multi-waste valorization approach.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A multi-waste valorization approach was proposed for bioethanol production. </LI> <LI> SSF of residues mixture was optimized with an industrial strain using high solids loading. </LI> <LI> High ethanol concentration was obtained by incorporating cheese whey in Eucalyptus SSF. </LI> <LI> <I>S. cerevisiae</I> Ethanol Red® was engineered for β-galactosidase production. </LI> <LI> Recombinant strain was used for ethanol production by SSF of residues mixture. </LI> </UL> </P>